Coding

Part:BBa_K1587003

Designed by: Mélissa David   Group: iGEM15_Toulouse   (2015-09-13)

3-hydroxybutyryl-CoA dehydratase

Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of Clostridium acetobutylicum. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA (Figure 1).

TLSE crt.jpg
Figure 1: Crotonyl-CoA formation catalyzed by Crt

We used BBa_K1587003 with other genes to produce butyrate in Escherichia coli (Figure 2).

TLSE ButMetabo.jpg
Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
chassisE.coli
protein3-hydroxybutyryl-CoA dehydratase